Phosphopeptide corresponding to amino acids 168–178 of human AMPK alpha
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Immunocytochemistry (ICC)
1 µg/ml
Immunofluorescence (IF)
1 µg/ml
Western Blot (WB)
2-5 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
AMPKa belongs to the family of AMP activated protein kinases, that plays a central role in regulating cellular and organismal energy balance in response to the balance between AMP/ATP, and intracellular Ca2+ levels. AMPK exists as a heterotrimer comprising of catalytic alpha subunit, and regulatory beta and gamma subunits. The alpha subunit is activated by increases in the cellular AMP:ATP ratio caused by metabolic stresses, and can mediate the effects of the adipokines leptin and adiponectin. Two isoforms differ in subcellular localization and AMP-dependence. Phosphorylation of threonine 172 in the alpha subunit is a key determinant of AMPK activity.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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