Phosphopeptide corresponding to amino acids 176–186 of human p38 MAPK
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
Western Blot (WB)
0.1-2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
p38 Mitogen-activated protein kinase (MAPK) belongs to the mitogen-activated protein kinase family, which responds to stress stimuli. The p38 MAPK subfamily plays an important role in cytokine production and the stress response. Members are activated by UV irradiation, heat shock, high osmotic stress, protein synthesis inhibitors, lipopolysaccharides, proinflammatory cytokines (e.g., IL-1 and TNF-alpha) and certain mitogens. There are four p38 kinases ( alpha, beta, lambda, and delta), with p38 alpha being well characterized, and expressed in most cell types. Additional functions for p38 MAPK include inhibition of cell cycle progression, development, and differentiation. An important target of p38 MAPK is the tumor suppressor protein p53, whose activation is positively regulated by p38. MKK3, MKK6 and SEK phosphorylate p38 MAPK at threonine 180 and tyrosine 182. Phosphorylation of these residues activates p38 MAPK, which in turn activates and phosphorylates ATF1, ATF2 and ATF6.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.