Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
3-5 ug per 1 x 10^6 cells
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
Western Blot (WB)
1-3 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with bovine, feline, porcine and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
FOXP3 (FORKHEAD BOX P3) belongs to the FOX protein family and the master regulator in T-Cell development and function. The gene encodes protein which is a member of the forkhead/winged-helix family of transcriptional regulators. It is shown that in human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage. FOXP3 is selectively expressed by a subset of CD4+ T cells, known as T regulatory cells, known to suppress T cell activity during response to foreign or self antigen selectively express FOXP3.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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