Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Western Blot (WB)
0.1-0.5 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Digoxigenin (DIG), a steroid, is a derivative of the flowers and leaves of plants – Digitalis. Due to the high immunigenicity of this small molecule it is highly preferred in many molecular biology applications. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications. The digoxigenin markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes for visualization and detection.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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