Protein corresponding to amino acids 1–268 of yeast Syntaxin.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
3-5 µg x 10^6 cells
Immunocytochemistry (ICC)
2-3 µg/ml
Immunofluorescence (IF)
2-3 µg/ml
Western Blot (WB)
0.1-1 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with Yeast based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Syntaxins belong to a family of SNARE proteins. The yeast Saccharomyces cerevisiae has about 7 genes similar to syntaxin and there are presently 15 types of syntaxin genes in the human genome. In presence of other cytoplasmic proteins like the N-ethylmaleimide-sensitive factor (NSF) and SNAP; synatxins can combine various vesicular transport systems for the exocytic and endocytic pathway. This is very important for the functioning and for bringing about specificity to the vesicular combination process.. The syntaxin protein in yeast is also known as the Pep12 protein. The function of this Pep12 protein in the yeast is to transport vesicles from the Golgi apparatus.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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