Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
1:100-1:500
Immunocytochemistry (ICC)
1:500-1:5000
Immunofluorescence (IF)
1:500-1:5000
Western Blot (WB)
1:500-1:5000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
c-Myc is a member of a family of closely related genes which also includes Nmyc, L-myc, P-myc, R-myc, S-myc, and B-myc. It was first identified as a protooncogene that was involved in the formation of leukemias. c-myc protein is involved in the control of cell proliferation, transformation and differentiation. This protein is expressed during embryonic development and is amplified and/or overexpressed in variety of tumors. The cellular localization of this protein is predominantly nuclear.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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