A recombinant protein corresponding to amino acids 1-142.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
1:100-1:500
Immunocytochemistry (ICC)
1:500-1:5000
Immunofluorescence (IF)
1:500-1:5000
Western Blot (WB)
1:500-1:5000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with bovine and hamster based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Survivin is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family and is also known as IAP4. The IAPs are involved in multiple cell functions such as cell signaling, cell division and metabolism. It exhibits differential expression in nearly all human cancers, but not in most normal tissues. Survivin has been shown to express in a cell cycle-dependent manner in the G2/M cell cycle and associates with the mitotic apparatus. The expression of surviving has been shown to be down regulated by expression of wild-type p53.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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