A recombinant protein corresponding to amino acids 109–266 of mouse IL-33.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Western Blot (WB)
0.1-1 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Interleukin 33 belongs to the IL-1 family of cytokines having dual mode of activity either as being secreted from cells to elicit their effects in an autocrine and paracrine manner or in an intracrine, nonsecreted manner as nuclear factors. IL-33 is produced by various tissue dwelling cells and is a crucial cytokine for Th2 cytokine-mediated host defense as well as induction of Th2-type allergic disorders. IL-33 has an additional role inside the cell, accumulating in the nucleus and associating with heterochromatin and mitotic chromatin and functioning as a transcriptional-repressor activity.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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