A peptide corresponding to amino acids 195-204 of Q9BUB5.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
0.5-1 ug/test
Immunocytochemistry (ICC)
4-6 ug/ml
Immunofluorescence (IF)
4-6 ug/ml
Immunohistochemistry (Paraffin) (IHC (P))
2-4 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, chimpanzee, equine, mouse, primate, rat, Rhesus monkey , porcine, Xenopus and zebrafish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
MAP kinase-interacting serine/threonine kinases (Mnk) function in signal transduction pathways through phosphorylation of eIF4E and are directly phosphorylated by ERK or p38 MAP kinases. In human and mouse there are two Mnk genes. Mnk1 is activated by phosphorylation at Thr197 and Thr202. Mnk1 and Mnk2 are essential for the phosphorylation of eIF4E at Ser209 however they differ markedly in their activity and regulatory patterns. Mnk1 is responsible for inducible phosphorylation of eIF4E while Mnk2 is responsible for basal levels of phosphorylation. Additionally, Mnk2 binds phosphorylated ERK while Mnk2 cannot. Mnk1 has been implicated in signaling in response to oxidative stress by increasing eIF4E phosphorylation upon H2O2 treatment in mice. Oxidative stress induced Mnk1 activity has also been linked to hyperproliferative diseases through the over-activation of eIF4E. Reactivity with Mnk2 has not been tested.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.