A peptide corresponding to amino acids 311-323 of P43403.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
1-3 ug/test
Immunocytochemistry (ICC)
1-3 ug/ml
Immunofluorescence (IF)
1-3 ug/ml
Western Blot (WB)
0.5-2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with primate, bovine and Xenopus based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Zeta-associated protein (ZAP-70), a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T-cell antigen receptor. Phosphorylation of both tyrosines 315. Phosphorylation of tyrosines 315 and 319 plays an important role in mediating the positive and negative selection of T cells in thymus. TLR9-activated B-cells display elevated ZAP-70 expression levels which correlate with sustained PKB induction. Additionally,, a major catechin found in green tea, binds directly to ZAP-70 resulting in caspase-mediated apoptosis in Jurkat cells.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.