Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
ELISA (ELISA)
1:100
Flow Cytometry (Flow)
3-5 µg/10^6 cells
Immunocytochemistry (ICC)
2-3 µg/mL
Immunofluorescence (IF)
2-3 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
1:100
Western Blot (WB)
2-3 µg/mL
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with bovine, feline, mouse and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
c-Met is tyrosine kinase receptor composed of a disulfide-linked heterodimer made of 45 kDa alpha-and 145 kDa beta-subunits found in many tissues. It expresses a transmembrane receptor-like protein, and is involved in regulating cellular proliferation, motility, morphogenesis, and apoptosis. c-Met encodes a high-affinity receptor for hepatocyte growth factor. Additionally, c-Met overexpression has been found in a wide variety of cancer types, particularly lung cancer.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.