643009 is specific for the heavy and light chains of rat IgG. The preparation has minimal cross reaction with mouse serum proteins, however, it may react with the light chains of other rat immunoglobulins.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
No
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
1/10 - 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light.
Shelf Life
18 months from the date of despatch.
Antiserum Preparation
Antiserum to rat IgG (H/L) was raised by repeated immunisation of donkeys with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.