Rabbit anti-Phosphotyrosine-HRP; HRP conjugated Polyclonal antibody specific to Multiple Phosphotyrosine [pY]. This antibody is validated for use in Western Blot, Immunoassay (ELISA).
Conjugate
HRP
Form
Liquid
Concentration
1 mg/ml
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 4mg/ml BSA, 40% glycerol
Preservative
0.1% Proclin 300
Storage Conditions
4° C
Tested Applications
Dilution *
ELISA (ELISA)
1:2000
Western Blot (WB)
1:1000-2000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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