6120-0004 is specific for Methotrexate and exhibits the following cross reactivities with related compounds:-
Aminopterin
18%
Folic acid
<0.01%
Dyhydrofolic acid
<0.01%
Tetrahydrofolic acid
<0.01%
N-5-Methyltetrahydrofolic acid
<0.01%
Folinic acid
<0.01%
N-(p-Aminobenzoyl)-L-glutamic acid
<0.01%
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.