5552-9009 is specific for the rod domain (aa 312-335) of human keratin 19. Keratin 19 is involved in the organization of myofibers. Together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin(1)
1/100 - 1/200
Western Blotting
(1)
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
In Western blotting 40 kD and 19 kD bands are observed.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
