5549-8006 recognizes both skeletal and corneal keratan sulphate chains either in isolated or proteoglycan-bound form.
The epitope is resistant to proteolytic or alkaline cleavages and partially sensitive to keratanase or other endo-B-galactosidase treatment.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Chicken
No
Dog
Yes
Mouse
Yes
Pig
Yes
Rat
No
Sheep
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/5,000 - 1/20,000
Radioimmunoassays
1/30,000 - 1/500,000
Western Blotting
1/1,000 - 1/4,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
May be used in a competitive inhibition assay to measure keratan sulphate levels. Dilutions must be determined by the end user.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
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