5345-0654 is recombinant human IGF-1 producd inE.coli.It exists as a single non-glycosylated polypeptide chain containing 70 amino acids.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at -70oC. For long term storage, the addition of a carrier protein is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
18 months from date of despatch.
Endotoxin Level
<0.1 ng/µg of IGF-1.
Peptide Sequence
The sequence of the first 5 N-terminal amino acids was found to be GPETL. N-terminal methionine has been completely removed enzymatically.