Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications.
This Opti-MEM® is modified as follows:
With
• GlutaMAX™
• Phenol Red
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains 查看更多insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Gibco® Opti-MEM® with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® Opti-MEM® product made in our Scotland facility (51985-026). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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