Recombinant protein derived from an internal region of the human NF-kappa-B (p50) (nuclear factor of kappa-B).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.25 mg/ml
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ELISA (ELISA)
0.1-1.0 ug/ml
Flow Cytometry (Flow)
3-5 µg/million cells
Gel Shift (GS)
5-10 ug/ml
Immunocytochemistry (ICC)
1µg/mL
Immunofluorescence (IF)
1µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
1:20-1:200
Western Blot (WB)
1.0 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
