Nerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.
This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus.
Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effec is 0.2-2.0 ng/ml.
Formulation: Lyophilized in 50 mM Na acetate buffer, pH 5.5, 150 mM NaCl, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Expiration Date: Expires one year from date of receipt when stored as instructed.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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