Insulin-Like Growth Factor Binding Protein 4 (IGFBP4), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications
This recombinant protein was expressed from a DNA sequence encoding the human IGFBP4 (NP_001543.2) precursor (Met 1-Glu 258) fused to a C-terminal polyhistidine tag.
Activity: Measured in a serumfree cell proliferation assay using MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. 1988, Cancer Research 48: 4083). The ED50 is typically 5-10 µg/ml.
Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Expiration Date: Expires one year from date of receipt when stored as instructed.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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