5022-1210 is specific for Huntingtin protein and reacts with an epitope between amino acids 181 and 810.
A band of approximately 350-400 kDa is observed in Western blotting.
Huntingtin is found in the perinuclear region along with microtubules, and in the centrosomal region along with gamma-tubulin. It is necessary for neuronal survival and is involved in synaptic vesicle trafficking, microtubule binding and may also have a role in apoptosis. In huntington disease, neuronal cells with the mutant form of huntingtin possess intranuclear aggregations of the N-terminal fragment, causing damaging inclusions in perinuclear locations and striatal neuron cell death. Wild-type huntington and anti-huntingtin reduce aggregation and cellular toxicity of the mutant huntingtin form in mammalian cell models of HD. Huntingtin is known to interact with GAPDH, HAP-1, SP1 and TAFII130.
Species Cross-Reactivity
Target Species
Cross Reactivity
Hamster
Yes
Monkey
Yes
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/500 - 1/5,000
Immunohistology - Frozen
1/500 - 1/5,000
Immunohistology - Paraffin(1)
1/500 - 1/5,000
Immunoprecipitation
1/500 - 1/5,000
Western Blotting
1/500 - 1/5,000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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