Cluster of Differentiation 4 (CD4), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 394) of mouse CD4 (NP_038516.1) fused to a polyhistidine tag at the C-terminus.
Activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells . When 5 x 104 cells/well are added to CD4 coated plates (2.5 µg/mland 100 µl/well), approximately 40 %-60 % will adhere specifically after 60 minutes at 37°C.
Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Expiration Date: Expires one year from date of receipt when stored as instructed.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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