4600-0004 recognises galactocerebroside, a surface antigen typically found on newly differentiated or developing oligodendrocytes. Staining of live cells prior to fixation will show a typical "patching" appearance. Cells fixed prior to staining will have a more solid, "smooth" appearance.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Expected from Sequence
Mouse
Yes
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/50 - 1/100
Immunofluorescence(1)
1/10 - 1/50
Immunohistology - Frozen(2)
1/10 - 1/50
Immunohistology - Paraffin
(1)
For staining of living cells it is recommended that the antisera be heat inactived prior to using to remove complement. The recommended fixative is 4% paraformaldehyde.
(2)
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. AbD Serotec recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
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