Truncated recombinant protein derived from human ELK1 protein
Conjugate
Unconjugated
Form
Frozen
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Tested Applications
Dilution *
Immunofluorescence (IF)
10 ug/ml
Immunoprecipitation (IP)
5 ug
Western Blot (WB)
2 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.