TAQMAN MIRNA ABC PURIF HUMAN PANEL A

产品编号：4473087 品牌：invitrogenGA 原厂货号：4473087
产品分类：DNA和RNA纯化 > RNA纯化 > microRNA和小RNA纯化
应用分类：

包装：

运保温度:

Store in refrigerator (2–8°C).

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

Use the TaqMan® miRNA ABC Purification Kit to purify high-quality miRNA from blood-related or other difficult sample types without hazardous chemicals.
• Simple and fast workflow using oligonucleotide-conjugated Dynal® magnetic beads
• Small sample input: typical volumes range from 10 µL for blood samples to 50 µL for serum and plasma samples
• RT-PCR inhibitors commonly found in blood-related samples are easily washed away
• No need for hazardous chemicals such as phenol, chloroform, Trizol® reagent, or guanidine isothiocyanate

Simple and Fast miRNA Purification
The TaqMan® miRNA ABC (anti-miRNA bead capture) purification kit contains buffers and magnetic beads for single-tube isolation of specific microRNA (miRNA) from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes (see workflow, Figure 1).

Human Panel A and Human Panel B
The TaqMan® miRNA ABC Purification Kit is offered in two configurations: Human Panel A and Human Panel B. The beads in each kit are superparamagnetic Dynabeads® covalently bound to a unique set of 377 anti-miRNA oligonucleotides for each panel that correspond to the assays contained in the TaqMan® Array Human MicroRNA A and B cards. The miRNA isolation process relies on hybridization of miRNAs to the corresponding anti-miRNA oligonucleotides attached to the beads. Complete lists of miRNAs isolated by the panels can be found in the TaqMan® miRNA ABC Purification Kit protocol (below).

miRNA Analysis
The miRNAs isolated using the TaqMan® miRNA ABC Purification Kit are ready for conversion into cDNA and downstream analysis by quantitative PCR using either single tube (individual) TaqMan® microRNA assays or the TaqMan® Array Human microRNA A and B cards. Figure 2 gives examples of the data that can be obtained from miRNA isolated from various sample types using the TaqMan® miRNA ABC Purification Kit.

注意事项：

For Research Use Only. Not for use in diagnostics procedures