4470-2189 is specific for human fibronectin and does not cross react with the following serum proteins: C-reactive protein, cytochrome C, fibrinogen, IgG, hSA, transferrin, haptoglobulin, a-1-antitrypsin, lactalbumin, a1-acid glycoprotein, antithrombin III, a-2-macro-globulin, plasminogen, ceruloplasmin or apolipoproteins A1, A2 or B.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Radial Immunodiffusion
Western Blotting
1/300 - 1/400
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.