The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene® Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.
• Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors
Maximize Total RNA Yields From Blood Samples The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from PAXgene® Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-18 µg from a single PAXgene® Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible.
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit performs exceptionally well at purifying miRNA with total RNA from PAXgene® Blood RNA Tubes without having to employ a separate miRNA protocol. One protocol does it all.
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from PAXgene® Blood RNA tubes as shown by TaqMan® gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.
Flexible Purification Formats The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods. • For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step. • For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process. • For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only).
Rapid Processing Time Processing times will vary based on format selected, but twelve PAXgene® Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.
An Alternative to Full Purification For users who perform primarily TaqMan®-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from PAXgene® Blood RNA tubes without conventional RNA purification.
One-Stop Shopping MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with our suite of reverse transcription mixes (SuperScript®), PCR master mixes (both TaqMan® and SYBR® based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.
Specifications: • Yield (Range): 3 - 18µg • Quality (RIN) ): ≥ 8 • Quality (A260⁄280) ): 1.8 – 2.2 • gDNA (w⁄w) ): <1.56% • Equipment Needed : *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with *50 mL conical tube adapters *50ml conical tubes *For tube format users: Tabletop microcentrifuge or picocentrifuge • Number of Preps: 96 preparations (blood tubes)
NOTE: Blood tubes and magnetic stands are sold separately.
NOTE: Blood tubes and magnetic stands are sold separately.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
PAXgene® and PreAnalytiX® are registered trademarks of PreAnalytiX GmbH. No sponsorship, endorsement, or affiliation is implied herein
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For Research Use Only. Not for use in diagnostic procedures.
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