The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 936. The sequence is conserved in cow.
Conjugate
Unconjugated
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol
Contains
0.05% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
3-5 µg/million cells
Immunocytochemistry (ICC)
1µg/mL
Immunofluorescence (IF)
1µg/mL
Western Blot (WB)
2-3 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1&2 signaling pathway.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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