The TaqMan® Array Rodent MicroRNA B Card v3.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 306 and 135 unique microRNAs for mouse and rat respectively in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls for each species are included to aid in data normalization and one TaqMan MicroRNA Assay not related to rodent is included as a negative control. Detail on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Rodent Pool B v3.0 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool B v3.0 for situations where sensitivity is of the utmost importance and/or sample is limiting.
TaqMan® Array MicroRNA Cards provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured microfluidic card, minimizing the experimental variability and effort required to run 384 TaqMan® MicroRNA Assays in parallel.
Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and are required to run the TaqMan® Array MicroRNA Card. For greater sensitivity, or when working with limited sample, Megaplex™ PreAmp Primers provide an optional preamplification step prior to real-time analysis. TaqMan® Array MicroRNA Cards and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with Sanger miRBase v14 (human) and v15 (rodent) to be created in as little as 5 hours, providing the ideal miRNA profiling solution. Key product features:
Highly specific—quantitate only the biologically active mature miRNAs
• Highly streamlined workflow reduces the number of RT (reverse transcription) reactions per sample • Simplify sample handling and increase sample throughput • Coverage for human, mouse and rat species • An ideal miRNA profiling solution
Simplify Sample Handling and Increase Sample Throughput Following reverse transcription of miRNA targets using Megaplex™ RT Primers and optional preamplification using Megaplex™ PreAmp Primers, TaqMan® Universal PCR Master Mix II is simply combined with each reaction and pipetted into each sample loading port in the TaqMan® Array Card, simplifying sample handling and increasing sample throughput.
Provides Comprehensive Coverage of Sanger miRBase Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan® Array Cards. The content is derived from miRBase v14 and v15 for human and rodent species respectively, providing close to full coverage of Sanger with the most up-to-date TaqMan® MicroRNA Assays.
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For Research Use Only. Not for use in diagnostic procedures.
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