TaqMan® Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). You can use TaqMan® Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.
This TaqMan® Universal Master Mix II is supplied as a 2X concentrate and contains:
Note: This TaqMan® Universal Master Mix II contains Uracil-N glycoslyase (UNG). See a complete listing of all TaqMan® Universal Master Mix II products, with and without UNG here.
TaqMan® Universal Master Mix II has been optimized for use with primers and TaqMan® probes that have been designed according to Life Technologies' guidelines. The master mix can be used with custom TaqMan® assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan® Gene Expression Assays • TaqMan® MicroRNA Assays • TaqMan® Drug Metabolism Genotyping Assays • TaqMan® SNP Genotyping Assays
For RNA quantitation experiments, the TaqMan® Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Life Technologies.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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