TAQMAN UNIVERSAL MMIX II NO UNG 5ML

产品编号：4440040 品牌：invitrogenGA 原厂货号：4440040
产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > qPCR
应用分类：实时定量 PCR > 实时定量PCR常见应用 > 基因表达分析
　　　　　实时定量 PCR > 实时定量PCR常见应用 > 小RNA与非编码RNA分析
　　　　　实时定量 PCR > 探针法RT-qPCR预混液

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4°C

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描述：

TaqMan^® Universal Master Mix II是适合多种TaqMan应用的实时荧光定量PCR预混液
► 室温下可在预先配置好的PCR反应体系中稳定保存24个小时
► 经验证可用于TaqMan^®基因表达、SNP基因分型、拷贝数和MicroRNA Assay
► 使用通用热循环条件运行TaqMan^® Assay
► 可直接替代您现有的实验方案

TaqMan^® Universal Master Mix II 可在较宽的靶基因输入量范围内完成敏感且精确的检测，它可以可靠地检测出低拷贝数靶点，并通过准确定量鉴别出靶点丰度之间的微小差异。与所有基于TaqMan^® Assay的技术一样，此实时荧光定量 PCR预混液提供了同源序列之间的单碱基分辨，且可在通用热循环条件下完成反应。

原厂资料：

TaqMan® Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). You can use TaqMan® Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan® Universal Master Mix II is supplied as a 2X concentrate and contains:

• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX™ Passive Reference
• Optimized buffer components

Note: This TaqMan® Universal Master Mix II does not contain Uracil-N glycoslyase (UNG). See a complete listing of all TaqMan® Universal Master Mix II products, with and without UNG here.

TaqMan® Universal Master Mix II has been optimized for use with primers and TaqMan® probes that have been designed according to Life Technologies' guidelines. The master mix can be used with custom TaqMan® assays available from our custom assay service, or with pre-optimized assays such as:

• TaqMan® Gene Expression Assays
• TaqMan® MicroRNA Assays
• TaqMan® Drug Metabolism Genotyping Assays
• TaqMan® SNP Genotyping Assays

For RNA quantitation experiments, the TaqMan® Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Life Technologies.