The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ETS2 that contains threonine 72.
Conjugate
Unconjugated
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol
Contains
0.05% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ChIP assay (ChIP)
1-5 µg
Flow Cytometry (Flow)
3-5 µg/million cells
Immunocytochemistry (ICC)
1-2 µg/ml
Immunofluorescence (IF)
1-2 µg/ml
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:50
Western Blot (WB)
1:500-1:2000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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