The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 100. The sequence is conserved in mouse and chicken.
Conjugate
Unconjugated
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol
Contains
0.05% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
3-5 µg x 10^6 cells
Immunocytochemistry (ICC)
2-3 µg/ml
Immunofluorescence (IF)
2-3 µg/ml
Western Blot (WB)
1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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