Ambion® validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs with the same chemical modifications for enhanced efficacy as in other Silencer® Select siRNAs.
• Validated siRNA controls for optimizing siRNA experiments
• GAPDH Positive Control functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells
What are Silencer® Select siRNAs? Silencer®Select siRNAs are the next generation Ambion® siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data. For more information, including data, 查看更多brochure, webinar, and FAQs, please visit applied biosystem's webiste.
Silencer® Select GAPDH Positive Control siRNA
Our extensively validated, positive control siRNA to human, mouse, and rat GAPDH serves multiple functions. First, it is an ideal "test" siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. In addition, because it targets GAPDH mRNA, which is commonly used as an internal control, its effects are easy to assay, and thus provide an excellent tool to monitor siRNA transfection efficiency by real-time RT-PCR, as well as with the Ambion® KDalert™ GAPDH Assay Kit. The Silencer® Select GAPDH Positive Control siRNA includes the same chemical modifications for reducing off-target effects as found in other Silencer® Select siRNAs.
Silencer® Select Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Applied Biosystems® has designed and extensively tested two Silencer® Select Negative Control siRNAs. These siRNAs include the same modifications for reducing off-target effects as found in other Silencer® Select siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these two controls have been tested in multi-parametric cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.
Quality Control
Applied Biosystems® synthesizes and purifies each Silencer® Select siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
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注意事项：
Upon receipt, store siRNAs in a non-frost-free freezer at or below –20 °.
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