Recombinant protein derived from the N-terminus of human Caspase 3 protein
Conjugate
Unconjugated
Form
Frozen
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Tested Applications
Dilution *
Flow Cytometry (Flow)
3-5 µg/million cells
Immunocytochemistry (ICC)
Assay Dependent
Immunofluorescence (IF)
Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:100
Immunoprecipitation (IP)
Assay Dependent
Western Blot (WB)
2mg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kDa and 12kDa, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.