Applied Biosystems® Power SYBR® Green预混液具有出色的核酸定量功能。Power SYBR®预混液还具有优秀的灵敏度及可重复性,可在不同模板浓度下对低至两个拷贝的目标基因进行检测。
• 通过单管形式,为您方便地提供了在基于SYBR® Green染料的PCR扩增及检测中所需的一切成分。Power SYBR® Green PCR预混液含有除模板及引物以外的所有组分,可助您进行基于SYBR® Green试剂的出色实时PCR实验。 • 经过优化的配方,其中包含了高度纯化的AmpliTaq Gold® LD型DNA聚合酶,可比经典的SYBR® Green PCR预混液提供更高的灵敏度。 • Power SYBR® Green PCR预混液含有dTTP/dUTP混合物,与AmpErase® UNG相兼容,可以最大限度减少残留污染。 • 含有专利版本的ROX™染料(作为内置被动参照),可以标准化与PCR无关的荧光波动从而最大限度减少孔间差异。这种差异可能由多种因素引起,如移液误差及样品蒸发等。 • 基于Power SYBR® Green试剂的PCR化学体系可以轻松地替代Applied Biosystems®实验方案中已有的SYBR® Green PCR预混液,直接使用相同的反应设置及热循环条件即可。
原厂资料:
The Power SYBR® Green PCR Master Mix is a convenient premix of the components (except primers, template, and water) necessary to perform real-time PCR using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR Green dye binds to double-stranded (ds) DNA, thus providing a fluorescent signal that reflects the amount of dsDNA product generated during PCR.
In RNA quantitation assays, you use the Power SYBR® Green PCR Master Mix in the second step of a two-step reverse-transcription polymerase chain reaction (RT-PCR) protocol. In a One-Step RT-PCR protocol, you add MultiScribe™ Reverse Transcriptase and RNase Inhibitor to the Power SYBR® Green PCR Master Mix.
You can use the Power SYBR Green PCR Master Mix with Applied Biosystems real-time PCR systems.
For the best quantitation results, use the following:
• Primer Express® software for primer design
• Applied Biosystems reagents
• Applied Biosystems universal thermal cycling conditions