Working concentrations for specific applications should be determined by the investigator. Appropriate dilutions will be
affected by several factors, including primary and secondary antibody affinity, antigen concentration and length of
incubations. We recommend the following ranges as starting points. Immunohistochemistry(1-6): 1:150 to 1:500
Western blot (chromagenic)(12): 1:1,250 to 1:5,000
Western blot (chemiluminescence)(8-11): 1:2,500 to 1:10,000
ELISA(13-16): 1:2,500 to 1:5,000
原厂资料:
反应性: HRP-SA 可与生物素反应。
应用: 免疫组织化学、Western 印迹和 ELISA
Working concentrations for specific applications should be determined by the investigator. Appropriate dilutions will be
affected by several factors, including primary and secondary antibody affinity, antigen concentration and length of
incubations. We recommend the following ranges as starting points. Immunohistochemistry(1-6): 1:150 to 1:500
Western blot (chromagenic)(12): 1:1,250 to 1:5,000
Western blot (chemiluminescence)(8-11): 1:2,500 to 1:10,000
ELISA(13-16): 1:2,500 to 1:5,000
注意事项:
For Research Use Only. Not for use in diagnostic procedures.