The BigDye Terminator v3.1 Cycle Sequencing Kit provides the required reagent components for the sequencing reaction in a ready reaction, pre-mixed format. You need only provide your template and the template-specific primer. These reagents are suitable for performing fluorescence-based cycle sequencing reactions on single-stranded or double-stranded DNA
templates, on polymerase chain reaction (PCR) fragments, and on large templates (for example, BAC clones).