4109-0004 reacts with an antigen associated with venous endothelial cells.
This antibody preferentially stains endothelial cells in medium and small veins, venules and capillaries in all organs. Negative, or weak staining is observed in large veins, arterioles and arteries. Lymphatic endothelium is also negative.
Species Cross-Reactivity
Target Species
Cross Reactivity
Goat
Yes
Pig
Yes
Rabbit
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunofluorescence
1/5 - 1/20
Immunohistology - Frozen
1/10 - 1/20
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.