Clone A10-33/1 recognizes endothelial cells in various cultures and organs (including lung, liver, gall bladder and skin). It is generally reactive with melanoma, including metastasizing melanomas and in carcinomas. It reacts strongly with purified GPIIb-IIIa which is expressed on platelets but not endothelial cells. Cross reactivity with nervous tissue has also been reported.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen(1)
1/100 - 1/400
Immunohistology - Paraffin
Western Blotting
(1)
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. AbD Serotec recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
The antigen is sensitive to formaldehyde. It is not suitable for use on paraffin embedded sections.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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