4060-1060 recognises alpha and beta human elastin. Elastin is a major structural protein expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus. It is also expressed in the large arteries, lung and skin. This antibody is also known to cross react with bovine alpha elastin.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin(1)
Western Blotting
(1)
Fixation with 10% formalin is recommended. Brief digestion with hyaluronidase or trypsin aids antigen retrieval.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
ELISA
In a simple ELISA, with 1 µg of native antigen coated per well, the 50% OD was observed at an antibody dilution of 1/8K. Under the same conditions, the end point was observed at 1/200K.
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