Synthetic peptide derived from an internal region of mouse ZONAB (ZO-1 associated nucleic acid-binding protein
Conjugate
Unconjugated
Form
Liquid
Concentration
0.25 mg/ml
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Immunofluorescence (IF)
2-3µg/ml
Immunohistochemistry (Frozen) (IHC (F))
2-3µg/ml
Immunoprecipitation (IP)
1-2µg
Western Blot (WB)
3-4µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with an internal region of the ZONAB protein. On Western blots, it identifies a major band at ~65 kDa and a minor band at ~54 kDa. The antibody is directed towards a region of ZONAB common to both the long and the alternatively-spliced short form. During development, reactivity was confirmed using mouse testis tissue homogenates by Western blotting and labeling of mouse testis tissue using immunofluorescence. All signals were absent in knockout mice, further confirming the reactivity with ZONAB.
Background/Target Information
ZONAB (ZO-1 associated nucleic acid-binding) is a Y-box transcription factor whose nuclear localization and transcriptional activity is regulated by the tight junction-associated candidate tumor suppressor ZO-1. Two ZONAB isoforms are known, a long isoform (ZONAB B) and an alternatively spliced shorter isoform (ZONAB A) that lacks 68 amino acids. ZONAB regulates the expression of the tyrosine kinase receptor HER2, and appears to act in combination with ZO-1 to regulate HER2 expression.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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