Reacts with the heavy and light chains of rabbit IgG and IgM and with the light chains of rabbit IgA as demonstrated by ELISA and flow cytometry.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
No
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
1/5 - 1/50
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to rabbit IgG/IgM were raised by repeated immunisations of goats with normal rabbit IgG and IgM.
The Ig fraction was purified by affinity chromatography on pooled rabbit IgG + IgM covalently linked to agarose.
The preparation has been cross absorbed with pooled human sera and purified human paraproteins to remove any potential cross reactivity.
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