Reacts with the heavy and light chains of rabbit IgG and IgM and with the light chains of rabbit IgA as demonstrated by ELISA and flow cytometry
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
No
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Neat - 1/10
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to rabbit IgG + IgM were raised by repeated immunisations of goats with normal rabbit IgG and IgM.
The Ig fraction was purified by affinity chromatography on pooled rabbit IgG + IgM covalently linked to agarose.
The preparation has been cross absorbed with pooled human sera and purified human paraproteins to remove any potential cross reactivity.
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