Recombinant protein corresponding to the N-terminal region of the human GAD65 protein
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ELISA (ELISA)
Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P))
1:10-1:50
Western Blot (WB)
1-3 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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