IgA (Immunoglobulin A) represents only 10-15% of the total Ig molecules in serum but is the predominating Ig isotype in saliva, tears, breast milk and in the gastrointestinal and urogenital tract. In serum, the IgA molecule is commonly found as a monomer of either IgA1 or IgA2 subclass but can also exist as dimers or larger polymeric forms. The IgA found in secretions (secretory IgA) is commonly a dimer with two IgA molecules joined by the J chain. Secretory IgA is the most important Ig isotype in mucous surfaces, the area of entry for most pathogens.
Application guide:
Monoclonal antibodies to human IgA
- for use in ELISpot (total IgA)
capture
MT57; purified
detection
MT20; biotinylated
- for use in ELISpot (ag specific IgA ELISpot with coated antigen)
detection
MT57; biotinylated
- for use in ELISpot (ag-specific IgA ELISpot with biotinylated antigen)
capture
MT57; purified
- for use in ELISA
capture
MT57; purified
detection
MT20; ALP conjugated
- for use in e.g. antigen-specific ELISA
detection
MT20; ALP conjugated
detection
MT20; biotinylated
Rat monoclonal antibody to human IgA, clone MT57.
Human IgA:
Suitable as capture mAb in ELISA in combination with mAb MT20-ALP (product code 3860-9A). Suitable as capture mAb in total IgA and antigen-specific IgA ELISpot. In total IgA ELISpot MT57 is recommended as capture mAb in combination with detection mAb MT20; biotinylated (product code 3860-6).
Monkey IgA:
Suitable as capture mAb in ELISA and ELISpot in combination with biotinylated goat anti-human IgA pAb (product code 3830-4)