IgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. The IgG molecule consists of two heavy and two lights chains (κ and λ) resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
ELISpot Plus kit for enumeration of total and antigen-specific human IgG producing cells.
The kit contains 4 ELISpot plates with PVDF membrane, capture mAbs, biotinylated detection mAbs, Streptavidin-ALP, polyclonal activators (R848 and recombinant human IL-2) and BCIP/NBT substrate..
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