IgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In mice the IgG subclasses are defined IgG1, IgG2a, IgG2b and IgG3. The IgG molecule consists of two heavy and two lights chains (κ and λ) resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
ELISpot Plus kit for enumeration of total and antigen-specific mouse IgG producing cells. The kit contains 4 ELISpot plates with PVDF membrane, capture Ab, biotinylated detection Ab, Streptavidin-ALP, polyclonal activators (R848 and recombinant mouse IL-2) and BCIP/NBT substrate.
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