Synthetic peptide containing six His residues (6xHis).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
Assay Dependent
Immunocytochemistry (ICC)
Assay Dependent
Immunofluorescence (IF)
Assay Dependent
Western Blot (WB)
1-5 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Background/Target Information
The His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. The His tag specific antibody is used to facilitate detection or coimmunopreciptation of tagged proteins.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.